Hi
I am running some DESeq2 code and I used the argument "counts(dds, normalized=TRUE)" to obtain the normalized table of counts. I understand that this normalization is taking into account length, RNA composition and deep sequencing.
I don't want to do differential expression. I want to do some regression and elastic net analyses. To do so, I need to work with the normalized counts. However, I don't know if I should apply a log2 to the normalized counts table before working with it, or if I can directly work with this data (if it already has log2, or if I don't need it). I just one to regress the outcome (logistic variable) to a continuous predictor variable (the gene obtained in the normalized counts table).
Do I have to apply log2 to the values of the normalized counts table?
Thanks!
