Why is RNA-Seq library size not constant among samples?
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FedeXandeR • 0
@federico-alessandro-25010
Last seen 10 days ago
Italy

Or "Everything You Always Wanted to Know About RNA-Seq (But Were Afraid to Ask) Part 1"

Dear all, I am afraid I am too much of a data analyst and too little wet (never seen a flowcell in my life) to understand the real reasons why the library size varies (even by millions of reads!) between samples sequenced on the same flow cell. Isn't megareads per sample a parameter decided at the machine level? Does this variability only have to do with multiplexing different samples on the same lane or is there more to it (amount of RNA or cDNA, PCR efficiency, sequencer intrinsic features)? Can anyone enlighten me about the different factors that affect this parameter, i.e., sample-specific sequencing depth?

Thank you so much.

[ crossposted on Biostars: https://www.biostars.org/p/9563303/ ]

RNA-Seq sequencer library_size sequency_depth multiplexing offtopic • 1.1k views
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This post does not fit the theme of this forum.

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Oops, I'm sorry. Should I remove this post?

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No, but I wouldn't really expect answers here.

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