Question

Sample sheet file for DiffBind

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Chris ▴ 20

@3fdb6f97

Last seen 3 months ago

United States

Hi all,

I got around 100k differential peaks which someone advices me there is something wrong in my ATAC-seq analyses, so I am trying to trace back. Is this sample sheet correct? Thank you so much. enter image description here

DiffBind • 864 views

ADD COMMENT • link updated 10 months ago by meghanapple2 • 0 • written 17 months ago by Chris ▴ 20

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I'm new to this so it looks a bit confusing for the world of mario, I'll look into it with more information.

ADD REPLY • link 16 months ago steffies127 • 0

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I don't know if there is something wrong but it's definitely interesting nerdle

ADD REPLY • link 10 months ago meghanapple2 • 0

score 2 · Answer 1 · 2023-06-26

There is nothing obviously incorrect about this sample sheet, so it looks like the experiment is set up correctly. The result is coming from the data. It is possible that there is a massive change in open chromatin, so getting 100k differential sites (split between both directions) is not definitely incorrect, only something unusual that should be validated if possible.