Hi, I need advice on how to set up the formula for the experimental design in DESeq2 in a complex analysis in which I have three DONORS and for each I have three TIMES and for each time I have an untreated control and a treated: DONOR1 TIME1 Control DONOR1 TIME1 Treated DONOR1 TIME2 Control DONOR1 TIME2 Treated DONOR1 TIME3 Control DONOR1 TIME3 Treated DONOR2 TIME1 Control DONOR2 TIME1 Treated DONOR2 TIME2 Control DONOR2 TIME2 Treated DONOR2 TIME3 Control DONOR2 TIME3 Treated DONOR3 TIME1 Control DONOR3 TIME1 Treated DONOR3 TIME2 Control DONOR3 TIME2 Treated DONOR3 TIME3 Control DONOR3 TIME3 Treated

the goal of the study is to find genes whose expression varies according to condition and time and independently of the donor. Please I would really appreciate your help. THANK YOU!

Ale