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Hello,
I am trying to read .fcs from a Sysmex Partec Cube 6 V2M flow cytometer, and get an error (with (R 4.2.1, flowCore2.10.0):
> library(flowCore)
> isFCSfile("AEboth")
AEboth
TRUE
> read.FCS("AEboth")
Error in readFCSdata(con, offsets, txt, transformation, which.lines, scale, :
object 'dat' not found
I am not expert enough to dig in the function code, and I do not see what is wrong with the .fcs. I can read it with other interfaces.
Thank you for your help,
Best,
Marie
Hi,
I don't know whether
traceback()
would give more details. I went throughread.FCS
code, but didn't get any clue.If you could share the file, it would be easier. My mail is on github if you need a private share.
I think this issue would deserve opening an issue on github https://github.com/RGLab/flowCore/issues
Best.
Thanks a lot for this quick answer.
traceback() returned:
I installed the package IFC to get get the function readFCSdata(), could not find it, but tried readFCS(), which works (problem solved for me) but with a warning:
I will submit this issue to the github, thanks for the suggestion
Best,
Marie
AEboth.fcs (FCS3.0) file