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Hello! I am having an error while doing normalization for my scRNAseq data, I would appreciate the help of anyone who countered the same problem the error is during quickcluster command as follow:
clust <- quickCluster(sce)
#output
Error in (function (A, nv = 5, nu = nv, maxit = 1000, work = nv + 7, reorth = TRUE, :
function 'as_cholmod_sparse' not provided by package 'Matrix
sessionInfo()
R version 4.3.2 (2023-10-31 ucrt)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 11 x64 (build 22621)
Matrix products: default
locale:
[1] LC_COLLATE=English_United States.utf8 LC_CTYPE=English_United States.utf8 LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C LC_TIME=English_United States.utf8
time zone: Europe/Berlin
tzcode source: internal
attached base packages:
[1] stats4 stats graphics grDevices utils datasets methods base
other attached packages:
[1] RSpectra_0.16-1 patchwork_1.1.3 BiocParallel_1.34.2 lubridate_1.9.3
[5] forcats_1.0.0 stringr_1.5.1 dplyr_1.1.4 purrr_1.0.2
[9] readr_2.1.4 tidyr_1.3.0 tibble_3.2.1 tidyverse_2.0.0.9000
[13] scater_1.28.0 ggplot2_3.4.4 scran_1.28.2 scuttle_1.10.2
[17] future_1.33.0 sctransform_0.4.1 Matrix_1.6-3 SingleCellExperiment_1.22.0
[21] SummarizedExperiment_1.30.2 Biobase_2.60.0 GenomicRanges_1.52.0 GenomeInfoDb_1.36.1
[25] IRanges_2.34.1 S4Vectors_0.38.1 MatrixGenerics_1.12.3 matrixStats_1.1.0
[29] AnnotationHub_3.8.0 BiocFileCache_2.8.0 dbplyr_2.3.4 BiocGenerics_0.46.0
loaded via a namespace (and not attached):
[1] rstudioapi_0.15.0 magrittr_2.0.3 ggbeeswarm_0.7.2 GenomicFeatures_1.52.2
[5] BiocIO_1.10.0 zlibbioc_1.46.0 vctrs_0.6.4 memoise_2.0.1
[9] Rsamtools_2.16.0 DelayedMatrixStats_1.22.6 RCurl_1.98-1.13 htmltools_0.5.7
[13] S4Arrays_1.0.5 progress_1.2.2 curl_5.1.0 BiocNeighbors_1.18.0
[17] parallelly_1.36.0 htmlwidgets_1.6.4 plyr_1.8.9 cachem_1.0.8
[21] GenomicAlignments_1.36.0 igraph_1.5.1 mime_0.12 lifecycle_1.0.4
[25] pkgconfig_2.0.3 rsvd_1.0.5 R6_2.5.1 fastmap_1.1.1
[29] GenomeInfoDbData_1.2.10 shiny_1.8.0 digest_0.6.33 colorspace_2.1-0
[33] AnnotationDbi_1.62.2 dqrng_0.3.2 irlba_2.3.5.1 RSQLite_2.3.3
[37] beachmat_2.16.0 filelock_1.0.2 fansi_1.0.5 timechange_0.2.0
[41] httr_1.4.7 abind_1.4-5 compiler_4.3.2 withr_2.5.2
[45] bit64_4.0.5 viridis_0.6.4 DBI_1.1.3 biomaRt_2.56.1
[49] MASS_7.3-60 rappdirs_0.3.3 DelayedArray_0.26.7 rjson_0.2.21
[53] bluster_1.10.0 tools_4.3.2 vipor_0.4.5 beeswarm_0.4.0
[57] interactiveDisplayBase_1.38.0 httpuv_1.6.12 future.apply_1.11.0 glue_1.6.2
[61] restfulr_0.0.15 promises_1.2.1 grid_4.3.2 cluster_2.1.4
[65] reshape2_1.4.4 generics_0.1.3 gtable_0.3.4 tzdb_0.4.0
[69] ensembldb_2.24.1 hms_1.1.3 metapod_1.8.0 BiocSingular_1.16.0
[73] ScaledMatrix_1.8.1 xml2_1.3.5 utf8_1.2.4 XVector_0.40.0
[77] ggrepel_0.9.4 BiocVersion_3.17.1 pillar_1.9.0 limma_3.56.2
[81] later_1.3.1 lattice_0.21-9 rtracklayer_1.60.1 bit_4.0.5
[85] tidyselect_1.2.0 locfit_1.5-9.8 Biostrings_2.68.1 gridExtra_2.3
[89] ProtGenerics_1.32.0 edgeR_3.42.4 statmod_1.5.0 DT_0.30
[93] stringi_1.8.2 lazyeval_0.2.2 yaml_2.3.7 codetools_0.2-19
[97] BiocManager_1.30.22 cli_3.6.1 xtable_1.8-4 munsell_0.5.0
[101] Rcpp_1.0.11 globals_0.16.2 png_0.1-8 XML_3.99-0.16
[105] parallel_4.3.2 ellipsis_0.3.2 blob_1.2.4 prettyunits_1.2.0
[109] AnnotationFilter_1.24.0 sparseMatrixStats_1.12.2 bitops_1.0-7 listenv_0.9.0
[113] viridisLite_0.4.2 scales_1.3.0 crayon_1.5.2 rlang_1.1.2
[117] KEGGREST_1.40.1
>
thank you in advance!
Ruba
I googled the error but didn't counter this one! it worked perfectly thank you for your help!