distanceToNearest
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@f0d6de0f
Last seen 10 months ago
United Kingdom

Hi,

I invoked the following command:

dist <- distanceToNearest(rt.gr, mop, ignore.strand = TRUE)

Note: rt.gr and mop are granges

I found out that NROW(dist) is one row shorter than NROW(rt.gr), meaning thate is one range in rt.gr that does not have a corresponding nearest range in mop. How do I find the missing row equivalent in rt.gr? Possibly did not return a value? I have checked ranges(rt.gr) for non-numeric values and did not find any.

Thanks

rtracklayer ranges distanceToNearest • 895 views
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Will you provide a self-contained example that shows the issue? It's hard to respond otherwise.

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After issuing this cmd:

dist <- distanceToNearest(rt.gr, mop, ignore.strand = TRUE)

I proceeded to do the following:

rt.gr$dat_dist <- mcols(dist)$distance

Then I got the following error:

Error in `[[<-`(`*tmp*`, name, value = c(3344L, 2127L, 24L, 183L, 255L,  : 
158611 elements in value to replace 158612 elements
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@james-w-macdonald-5106
Last seen 14 hours ago
United States

You shouldn't expect that the length of dist will be the same as the length as your rt.gr object. Also, by 'self-contained' example, I meant something that other people could run. Just showing your code is not self-contained because I don't have your data. But here is an example.

## run this first
> example(distanceToNearest) 
> query
GRanges object with 2 ranges and 0 metadata columns:
      seqnames    ranges strand
         <Rle> <IRanges>  <Rle>
  [1]        A         1      *
  [2]        B         5      *
  -------
  seqinfo: 2 sequences from an unspecified genome; no seqlengths
> subject
GRanges object with 3 ranges and 0 metadata columns:
      seqnames    ranges strand
         <Rle> <IRanges>  <Rle>
  [1]        A      6-10      *
  [2]        A      5-10      *
  [3]        A     13-15      *
  -------
  seqinfo: 1 sequence from an unspecified genome; no seqlengths

## this will return a Hits object that conforms to the 'subject' GRanges object
> distanceToNearest(subject, query)
Hits object with 3 hits and 1 metadata column:
      queryHits subjectHits |  distance
      <integer>   <integer> | <integer>
  [1]         1           1 |         4
  [2]         2           1 |         3
  [3]         3           1 |        11
  -------
  queryLength: 3 / subjectLength: 2

## this will return a Hits object that DOES NOT conform to the 'query' GRanges object.
> distanceToNearest(query,subject)
Hits object with 1 hit and 1 metadata column:
      queryHits subjectHits |  distance
      <integer>   <integer> | <integer>
  [1]         1           2 |         3
  -------
  queryLength: 2 / subjectLength: 3
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But do note that

> z <- distanceToNearest(query,subject)
> query$distance <- NA
> query$distance[queryHits(z)] <- mcols(z)$distance
> query
GRanges object with 2 ranges and 1 metadata column:
      seqnames    ranges strand |  distance
         <Rle> <IRanges>  <Rle> | <integer>
  [1]        A         1      * |         3
  [2]        B         5      * |      <NA>
  -------
  seqinfo: 2 sequences from an unspecified genome; no seqlengths
>
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Also with plyranges:

> query %>% add_nearest_distance(subject)
GRanges object with 2 ranges and 1 metadata column:
      seqnames    ranges strand |  distance
         <Rle> <IRanges>  <Rle> | <integer>
  [1]        A         1      * |         3
  [2]        B         5      * |      <NA>
  -------
  seqinfo: 2 sequences from an unspecified genome; no seqlengths
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Thanks Michael. It's good to know this alternative way.

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Thanks James. I really appreciate your explanations.

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