seqlevelsStyle() not working
2
0
Entering edit mode
Kai Hu ▴ 70
@kai
Last seen 6 months ago
Worcester

I got the following error when invoking seqlevelsStyle():

 seqlevelsStyle(annoData) <- "UCSC"

Error in download.file(url, destfile, quiet = TRUE) : 
  cannot open URL 'https://hgdownload.cse.ucsc.edu/goldenPath/hg19/database/chromInfo.txt.gz'

Seems that the URL is no longer valid, and has been replaced with: https://hgdownload2.cse.ucsc.edu/goldenPath/hg19/database/chromInfo.txt.gz

The following link seems to work as well: https://hgdownload2.soe.ucsc.edu/goldenPath/hg19/database/chromInfo.txt.gz

Any input on how to solve this and which link should be used for downloading?

ensembldb genomei GenomeInfoDb • 947 views
ADD COMMENT
2
Entering edit mode
@kentriemondy-14219
Last seen 4 months ago
Denver, University of Colorado Anschutz…

An update to the GenomeDbInfo package was pushed to Bioconductor 3.18 recently that should fix this and will be available in a day or two. In the meantime you can change the domain used by seqlevelsStyle() by setting this option in your session:

options(UCSC.goldenPath.url="https://hgdownload.soe.ucsc.edu/goldenPath")
ADD COMMENT
0
Entering edit mode

Ty, this works for me!

ADD REPLY
0
Entering edit mode
@james-w-macdonald-5106
Last seen 2 days ago
United States

Do you need to update your versions of R/Bioconductor?

> gr <- GRanges("1:100-1000")
> seqlevelsStyle(gr) <- "UCSC"
> gr
GRanges object with 1 range and 0 metadata columns:
      seqnames    ranges strand
         <Rle> <IRanges>  <Rle>
  [1]     chr1  100-1000      *
  -------
  seqinfo: 1 sequence from an unspecified genome; no seqlengths
> sessionInfo()
R version 4.3.1 (2023-06-16 ucrt)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 11 x64 (build 22621)

Matrix products: default


locale:
[1] LC_COLLATE=English_United States.utf8 
[2] LC_CTYPE=English_United States.utf8   
[3] LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.utf8    

time zone: America/New_York
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics 
[4] grDevices utils     datasets 
[7] methods   base     

other attached packages:
[1] GenomicFeatures_1.54.1
[2] AnnotationDbi_1.64.1  
[3] Biobase_2.62.0        
[4] GenomicRanges_1.54.1  
[5] GenomeInfoDb_1.38.5   
[6] IRanges_2.36.0        
[7] S4Vectors_0.40.2      
[8] BiocGenerics_0.48.1   

loaded via a namespace (and not attached):
 [1] KEGGREST_1.42.0            
 [2] SummarizedExperiment_1.32.0
 [3] rjson_0.2.21               
 [4] lattice_0.22-5             
 [5] vctrs_0.6.5                
 [6] tools_4.3.1                
 [7] bitops_1.0-7               
 [8] generics_0.1.3             
 [9] curl_5.2.0                 
[10] parallel_4.3.1             
[11] tibble_3.2.1               
[12] fansi_1.0.6                
[13] RSQLite_2.3.5              
[14] blob_1.2.4                 
[15] pkgconfig_2.0.3            
[16] Matrix_1.6-5               
[17] dbplyr_2.4.0               
[18] lifecycle_1.0.4            
[19] GenomeInfoDbData_1.2.11    
[20] compiler_4.3.1             
[21] stringr_1.5.1              
[22] Rsamtools_2.18.0           
[23] Biostrings_2.70.2          
[24] progress_1.2.3             
[25] codetools_0.2-19           
[26] RCurl_1.98-1.14            
[27] yaml_2.3.8                 
[28] pillar_1.9.0               
[29] crayon_1.5.2               
[30] BiocParallel_1.36.0        
[31] DelayedArray_0.28.0        
[32] cachem_1.0.8               
[33] abind_1.4-5                
[34] tidyselect_1.2.0           
[35] digest_0.6.34              
[36] stringi_1.8.3              
[37] dplyr_1.1.4                
[38] restfulr_0.0.15            
[39] grid_4.3.1                 
[40] biomaRt_2.58.1             
[41] fastmap_1.1.1              
[42] SparseArray_1.2.3          
[43] cli_3.6.2                  
[44] magrittr_2.0.3             
[45] S4Arrays_1.2.0             
[46] XML_3.99-0.16.1            
[47] utf8_1.2.4                 
[48] prettyunits_1.2.0          
[49] filelock_1.0.3             
[50] rappdirs_0.3.3             
[51] bit64_4.0.5                
[52] XVector_0.42.0             
[53] httr_1.4.7                 
[54] matrixStats_1.2.0          
[55] bit_4.0.5                  
[56] png_0.1-8                  
[57] hms_1.1.3                  
[58] memoise_2.0.1              
[59] BiocIO_1.12.0              
[60] BiocFileCache_2.10.1       
[61] rtracklayer_1.62.0         
[62] rlang_1.1.3                
[63] glue_1.7.0                 
[64] DBI_1.2.1                  
[65] xml2_1.3.6                 
[66] R6_2.5.1                   
[67] MatrixGenerics_1.14.0      
[68] GenomicAlignments_1.38.2   
[69] zlibbioc_1.48.0            
>

Login before adding your answer.

Traffic: 817 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6