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Hi,
I have 6 samples/ groups with 3 replicates (In total 17; one sample contains 2 replicates). the sample names are Mf, Bf, Ef, Mr, Br, and Er. Suppose Mf, Bf, and Ef are coming from control and Mr, Br, and Er are coming from treatment.
How to make DGEList and design matrix for that. I want to get DEGs (differentially expressed genes) between Mr-Mf, Br-Bf, Er-Ef.
Thank you in advance for your input.
Thank you for your suggestion. Yes, I have made DGEList and design matrix in my script. But I was not entirely confident while making those, as I am new in transcriptomic data analysis.