How to make DGEList and design matrix by edgeR package
1
0
Entering edit mode
prity6459 • 0
@84d63c4c
Last seen 6 months ago
France

Hi,

I have 6 samples/ groups with 3 replicates (In total 17; one sample contains 2 replicates). the sample names are Mf, Bf, Ef, Mr, Br, and Er. Suppose Mf, Bf, and Ef are coming from control and Mr, Br, and Er are coming from treatment.

How to make DGEList and design matrix for that. I want to get DEGs (differentially expressed genes) between Mr-Mf, Br-Bf, Er-Ef.

Thank you in advance for your input.

DGEList edgeR • 365 views
ADD COMMENT
0
Entering edit mode
@gordon-smyth
Last seen 1 hour ago
WEHI, Melbourne, Australia

Your question is curious because you showed code that made a DGEList and design matrix in your previous post just 15 hours ago.

Please have a read of the edgeR User's Guide.

ADD COMMENT
0
Entering edit mode

Thank you for your suggestion. Yes, I have made DGEList and design matrix in my script. But I was not entirely confident while making those, as I am new in transcriptomic data analysis.

ADD REPLY

Login before adding your answer.

Traffic: 966 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6