Hello,

What is the most appropriate way to calculate Cohen's D while using DESeq2 package for differential expression analysis?

Can one calculate it using log2FC and lfcSE from summary stats as follows: log2FC/(lfcSE*sqrt(sample_size)) ?

We found that the abs(Cohen'sD) values are always less than 0.6 using the above approach.

Any feedback would be great!

Thank you!

Thank you for your reply. We will follow this guidance.