Using Normalized Counts in DESeq2
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KokiBio • 0
@a7f26943
Last seen 1 day ago
United States

Hello,

I am wondering if there is any way I can use normalized counts in DESeq2. For context, access to the RNA-seq data I need on TCGA is restricted, so obtaining the raw files will take a while. In the mean time, I want to perform exploratory analysis and see how the data looks. I understand that raw files are the standard for publishing.

Thank you!

DESeq2 • 173 views
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ATpoint ★ 4.5k
@atpoint-13662
Last seen 1 hour ago
Germany

TCGA raw counts can be obtained for example using the TCGAbiolinks or recount packages.

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Also, just to add, there are many dozens of posts on this topic of DESeq2 applied to TCGA counts, normalized or other. Recommend google search with site:support.bioconductor.org

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