Diffbind Analysis gives error during profile plotting stage. Error in value[[3L]](cond) : profileplyr error: Error: BiocParallel errors 1 remote errors, element index: 1 5 unevaluated and other
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Muhammad • 0
@8bf8526f
Last seen 4 months ago
Sweden

I am running Diffbind for a chipseq dataset for the last 2 years or so. I did'nt had any problem before, though i am not very expert. Now when i am running Diffind, it gives me error during ploting profiles. when i run


profiles <- dba.plotProfile(DiffPeaks)

dba.plotProfile(profiles)

it gives me this error

Error in value[[3L]](cond) : 
  profileplyr error: Error: BiocParallel errors
  1 remote errors, element index: 1
  5 unevaluated and other errors
  first remote error:
Error: BiocParallel errors
  1 remote errors, element index: 1
  0 unevaluated and other errors
  first remote error:
Error in DataFrame(..., check.names = FALSE): different row counts implied by arguments

the bam and peak files seems OK, hrere is the session info. I have tried to uninstall R and re-install it updating packages but no luck

R version 4.5.0 (2025-04-11 ucrt)
Platform: x86_64-w64-mingw32/x64
Running under: Windows 11 x64 (build 26100)

Matrix products: default
  LAPACK version 3.12.1

locale:
[1] LC_COLLATE=English_United States.utf8  LC_CTYPE=English_United States.utf8    LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C                           LC_TIME=English_United States.utf8    

time zone: Europe/Berlin
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] BiocParallel_1.42.0         here_1.0.1                  profileplyr_1.24.1          DiffBind_3.18.0            
 [5] SummarizedExperiment_1.38.1 Biobase_2.68.0              MatrixGenerics_1.20.0       matrixStats_1.5.0          
 [9] GenomicRanges_1.60.0        GenomeInfoDb_1.44.0         IRanges_2.42.0              S4Vectors_0.46.0           
[13] BiocGenerics_0.54.0         generics_0.1.4              BiocManager_1.30.25        

loaded via a namespace (and not attached):
  [1] later_1.4.2                               splines_4.5.0                            
  [3] BiocIO_1.18.0                             bitops_1.0-9                             
  [5] ggplotify_0.1.2                           tibble_3.2.1                             
  [7] R.oo_1.27.1                               preprocessCore_1.70.0                    
  [9] XML_3.99-0.18                             lifecycle_1.0.4                          
 [11] pwalign_1.4.0                             mixsqp_0.3-54                            
 [13] rprojroot_2.0.4                           doParallel_1.0.17                        
 [15] lattice_0.22-7                            MASS_7.3-65                              
 [17] systemPipeR_2.14.0                        magrittr_2.0.3                           
 [19] limma_3.64.0                              yaml_2.3.10                              
 [21] plotrix_3.8-4                             httpuv_1.6.16                            
 [23] ggtangle_0.0.6                            cowplot_1.1.3                            
 [25] DBI_1.2.3                                 RColorBrewer_1.1-3                       
 [27] abind_1.4-8                               ShortRead_1.66.0                         
 [29] purrr_1.0.4                               R.utils_2.13.0                           
 [31] RCurl_1.98-1.17                           yulab.utils_0.2.0                        
 [33] circlize_0.4.16                           GenomeInfoDbData_1.2.14                  
 [35] enrichplot_1.28.2                         ggrepel_0.9.6                            
 [37] irlba_2.3.5.1                             tidytree_0.4.6                           
 [39] pheatmap_1.0.12                           ChIPseeker_1.44.0                        
 [41] codetools_0.2-20                          DelayedArray_0.34.1                      
 [43] DOSE_4.2.0                                DT_0.33                                  
 [45] tidyselect_1.2.1                          shape_1.4.6.1                            
 [47] aplot_0.2.5                               UCSC.utils_1.4.0                         
 [49] farver_2.1.2                              GenomicAlignments_1.44.0                 
 [51] jsonlite_2.0.0                            GetoptLong_1.0.5                         
 [53] iterators_1.0.14                          foreach_1.5.2                            
 [55] bbmle_1.0.25.1                            progress_1.2.3                           
 [57] tools_4.5.0                               treeio_1.32.0                            
 [59] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2   snow_0.4-4                               
 [61] Rcpp_1.0.14                               glue_1.8.0                               
 [63] SparseArray_1.8.0                         DESeq2_1.48.1                            
 [65] qvalue_2.40.0                             amap_0.8-20                              
 [67] dplyr_1.1.4                               withr_3.0.2                              
 [69] numDeriv_2016.8-1.1                       fastmap_1.2.0                            
 [71] latticeExtra_0.6-30                       boot_1.3-31                              
 [73] caTools_1.18.3                            digest_0.6.37                            
 [75] truncnorm_1.0-9                           mime_0.13                                
 [77] R6_2.6.1                                  gridGraphics_0.5-1                       
 [79] colorspace_2.1-1                          GO.db_3.21.0                             
 [81] GreyListChIP_1.40.0                       gtools_3.9.5                             
 [83] jpeg_0.1-11                               RSQLite_2.3.11                           
 [85] R.methodsS3_1.8.2                         tidyr_1.3.1                              
 [87] data.table_1.17.0                         rtracklayer_1.68.0                       
 [89] prettyunits_1.2.0                         httr_1.4.7                               
 [91] htmlwidgets_1.6.4                         S4Arrays_1.8.0                           
 [93] org.Mm.eg.db_3.21.0                       pkgconfig_2.0.3                          
 [95] gtable_0.3.6                              blob_1.2.4                               
 [97] ComplexHeatmap_2.24.0                     hwriter_1.3.2.1                          
 [99] XVector_0.48.0                            chipseq_1.58.0                           
[101] htmltools_0.5.8.1                         fgsea_1.34.0                             
[103] clue_0.3-66                               scales_1.4.0                             
[105] TxDb.Hsapiens.UCSC.hg38.knownGene_3.21.0  png_0.1-8                                
[107] ashr_2.2-63                               ggfun_0.1.8                              
[109] reshape2_1.4.4                            rjson_0.2.23                             
[111] coda_0.19-4.1                             nlme_3.1-168                             
[113] curl_6.2.2                                bdsmatrix_1.3-7                          
[115] org.Hs.eg.db_3.21.0                       cachem_1.1.0                             
[117] GlobalOptions_0.1.2                       stringr_1.5.1                            
[119] KernSmooth_2.23-26                        parallel_4.5.0                           
[121] AnnotationDbi_1.70.0                      restfulr_0.0.15                          
[123] apeglm_1.30.0                             pillar_1.10.2                            
[125] grid_4.5.0                                vctrs_0.6.5                              
[127] gplots_3.2.0                              promises_1.3.2                           
[129] cluster_2.1.8.1                           xtable_1.8-4                             
[131] rGREAT_2.10.0                             EnrichedHeatmap_1.38.0                   
[133] invgamma_1.1                              GenomicFeatures_1.60.0                   
[135] mvtnorm_1.3-3                             cli_3.6.5                                
[137] locfit_1.5-9.12                           compiler_4.5.0                           
[139] Rsamtools_2.24.0                          rlang_1.1.6                              
[141] crayon_1.5.3                              SQUAREM_2021.1                           
[143] labeling_0.4.3                            interp_1.1-6                             
[145] emdbook_1.3.13                            plyr_1.8.9                               
[147] fs_1.6.6                                  stringi_1.8.7                            
[149] TxDb.Mmusculus.UCSC.mm10.knownGene_3.10.0 deldir_2.0-4                             
[151] Biostrings_2.76.0                         tiff_0.1-12                              
[153] lazyeval_0.2.2                            GOSemSim_2.34.0                          
[155] Matrix_1.7-3                              BSgenome_1.76.0                          
[157] hms_1.1.3                                 patchwork_1.3.0                          
[159] bit64_4.6.0-1                             ggplot2_3.5.2                            
[161] shiny_1.10.0                              KEGGREST_1.48.0                          
[163] statmod_1.5.0                             TxDb.Mmusculus.UCSC.mm9.knownGene_3.2.2  
[165] igraph_2.1.4                              memoise_2.0.1                            
[167] ggtree_3.16.0                             fastmatch_1.1-6                          
[169] soGGi_1.40.1                              bit_4.6.0

Any help will be appreciated Thanks

chipseq profileplyr DiffBind • 956 views
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