sigDF object in sesame
0
0
Entering edit mode
Ramiro • 0
@8f137d25
Last seen 22 days ago
United States

I am interested in obtaining the methylated and unmethylated signal intensities. I am using the sesame package, and obtained the sigDF object as follows:

sdfs = openSesame(baseDirectory,prep="QCDPB",func=NULL,BPPARAM = BiocParallel::MulticoreParam(4),platform = "EPICv2")

I get, for the first sample something like this:

Probe_ID                MG MR      UG        UR         col  mask \
cg00000029_TC21 NA NA 2709.775 1046.9992   2 FALSE 
cg00000109_TC21 NA NA 2770.632  288.9767   2 FALSE 
cg00000155_BC21 NA NA 3668.775 230.5298   2 FALSE

Can't find the definitions anywhere but I am guessing that MG, MR, UG and UR are what I am looking for (methylated green, methylated red, unmethylated green and unmethylated red). However, it seems that MG and MR do not have a lot of data:

sum(!is.na(sdf[["sample1"]]$MG))

[1] 128295

sum(!is.na(sdf[["sample1"]]$UG))

[1] 937688

Is there something that we are missing or does this look normal and correspond to the methylated and unmethylated intensities?

sesame sesameData • 88 views
ADD COMMENT

Login before adding your answer.

Traffic: 564 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6