Post translational modifications and phosphoproteomics in limpa?
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@26240b78
Last seen 15 hours ago
United States

My group's started using limpa and we're considering adopting it for all our proteomics analyses. We have some data with PTMs (most commonly phosphorylation) - is there a way to study these protein modifications with limpa or use limpa's methods as part of such an analysis? Thanks for the package!

limpa • 35 views
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@gordon-smyth
Last seen 2 hours ago
WEHI, Melbourne, Australia

Yes, we use limpa for PTMs ourselves. I assume your data is preprocessed so that each row corresponds to a PTM. You replace dpcQuant() with dpcImpute(), to tell limpa you want to keep each row separate instead of summarizing at the protein level. Otherwise the whole pipeline is unchanged.

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