> sessionInfo()
R version 3.3.2 (2016-10-31)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows >= 8 x64 (build 9200)
locale:
[1] LC_COLLATE=German_Germany.1252 LC_CTYPE=German_Germany.1252 LC_MONETARY=German_Germany.1252 LC_NUMERIC=C
[5] LC_TIME=German_Germany.1252
attached base packages:
[1] stats4 parallel stats graphics grDevices utils datasets methods base
other attached packages:
[1] rtracklayer_1.34.2 CrispRVariants_1.2.0 ggplot2_2.2.1 Rsamtools_1.26.1 Biostrings_2.42.1 XVector_0.14.0
[7] GenomicRanges_1.26.3 GenomeInfoDb_1.10.3 IRanges_2.8.1 S4Vectors_0.12.1 BiocGenerics_0.20.0 BiocInstaller_1.24.0
loaded via a namespace (and not attached):
[1] Rcpp_0.12.9 plyr_1.8.4 bitops_1.0-6 tools_3.3.2
[5] zlibbioc_1.20.0 digest_0.6.12 RSQLite_1.1-2 memoise_1.0.0
[9] tibble_1.2 gtable_0.2.0 lattice_0.20-34 Matrix_1.2-8
[13] DBI_0.5-1 gridExtra_2.2.1 stringr_1.2.0 grid_3.3.2
[17] Biobase_2.34.0 AnnotationDbi_1.36.2 XML_3.98-1.5 BiocParallel_1.8.1
[21] reshape2_1.4.2 magrittr_1.5 scales_0.4.1 GenomicAlignments_1.10.0
[25] assertthat_0.1 SummarizedExperiment_1.4.0 colorspace_1.3-2 stringi_1.1.2
[29] RCurl_1.95-4.8 lazyeval_0.2.0 munsell_0.4.3
> crispr_set <- readsToTarget(reads = "subsample.bam" , target = gr, reference = reference, target.loc = 200)
Read 694 alignments, excluded 0
0 from 692 (0.00%) chimeras did not involve guide
692 from 692 (100.00%) remaining chimeric reads included
0 (0.00%) assigned to more than one target
0 of 2 nonchimeric reads span the target range
Initialising CrisprRun subsample.bam
Initialising CrisprSet lenti_zeo_self_targeting:4840-5460 with 1 samples
Error in .self$.genomeToTargetLocs(target.loc, start(target), end(target), :
unused argument (rc = rc)
Would someone be so kind to explain to me what this error message means?
