Dear tRanslatome developers,

We would like to compare the transcriptome and translatome after pathogen treatment of Arabidopsis WT and Knock-Out-Line for a RNA-binding protein that leads to reduced translational activity for specific targets.

Due to experimental reasons, we applied different input concentrations of total RNA and polyrib. RNA (100/150 ng) to Affymetrix arrays. I would like to know how to normalize the data before using the rTranslatome package. It is possible to merge both datasets and apply quantile normalize? If not, do you have any suggestions to process the data properly (other normalization, statistical tests) ?

Best regards,

Benedikt