Hello,
I have gated for CD14 cells from my raw FCS data, and am now trying to apply a gate to the isolated CD14 cells to isolate HLA-DR+ monocytes. When trying to apply a gate in openCyto, however, the program adds the dims from my gating template as populations:
> gt<-gatingTemplate("/Users/justinyi/Desktop/gating_template.csv")
Adding population:monocyte
Adding population:BL4-H+
Adding population:SSC-H+
Adding population:HLA-DR
and I receive an error when trying to gate the isolated monocyte population:
Error in (function (fs, pp_res, gFunc, popAlias, channels, gFunc_args): failed at A1_11.fcs
Error in .find_peaks(x, ...)[, "x"] : incorrect number of dimensions
Here is my gating template. If someone could help me resolve this issue that would be excellent
| alias |
pop |
parent | dims | gating_method | gating_args | collapseDataForGating | groupBy | preprocessing_method | preprocessing_args |
| CD14 | CD14 | root | FSC-H,SSC-H | flowClust | K=3,target=c(1e6,2e5),quantile=0.25 | NA | NA | NA | NA |
| HLA-DR+ | HLA-DR+ | CD14 | BL4-H,SSC-H | mindensity | gate_range=c(1.5e1,1.5e4) | NA | NA | NA | NA |
