I am very new to bioinformatic analyses and have learned how to do the basic ChIP-seq workflow in Galaxy, including using Bowtie2 to map reads to the genome, MACS2 to call peaks, and doing motif searching.
Now I'd like to learn how to identify sites that are deferentially bound between two sample groups. I've read through the Bioconductor manual for Diffbind, but I do not understand much beyond the Introduction section.
I've installed R and RStudio, but am not knowledgeable in programming languages. I'm wondering how much I need to learn to be able to perform Diffbind on my ChIP-seq datasets.
Are there any Diffbind tutorials/guides for complete newbs?
Great, thank you.