Hello,

I tried DiffBind (v3.0.9) to find DE binding sites from ChIP-seq data. When I used "dba.normalize" with "background=TRUE". It ended up with this error:

DB = dba.normalize(DB, normalize=DBA_NORM_NATIVE, background=TRUE)
Generating background bins...
Error in .local(x, ...) : 
  cannot create 160 workers; 125 connections available in this session 

sessionInfo( )

It seems that my server has 160 cpu cores, and BiocParallel or parallel embeded in DiffBind aquire all the cpu cores in running the task, while the R session only support up to 125 cores.

I'm woundering if you can provide any solution to this issue?

Thanks very much!

WS

You can set the number of cores that DiffBind uses as a configuration option before calling dba.normalize():

DB$config$cores <- 32