User: vlaufer

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vlaufer0
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Posts by vlaufer

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Using Effect Coding or Orthogonal Coding instead of dummy coding in DESeq2?
... I have a balanced study design including two variables, genotype and location. I have a research question of the following form: "Does genotype status (knockout vs wildtype) affect gene expression in intestinal surface cells differently than it affects intestinal crypt cells" For this, my proposed ...
deseq2 interactions multiple factor design orthogonal coding effect coding written 18 months ago by vlaufer0 • updated 18 months ago by Peter Langfelder1.9k
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Comment: C: Please help me verify my compensation and spill matrices makes sense ( openCyto
... Thank you. I am a bit worried that the 1's are off of the diagonal ... ...
written 19 months ago by vlaufer0
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Please help me verify my compensation and spill matrices makes sense ( openCyto package, in flowCore )
... The code below makes a flowSet object, then trims out columns as described in other posts at this forum. Following this, I use the spillover command to create my compensation matrix as detailed here: https://support.bioconductor.org/p/51931/ # open .FCS files fcs_names <- list.files(fcspath, p ...
flowcore flow cytometry spillover compensation matrix written 19 months ago by vlaufer0 • updated 19 months ago by SamGG180

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