User: Marcelo Laia

gravatar for Marcelo Laia
Marcelo Laia430
Reputation:
430
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Location:
Brazil
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Last seen:
6 months ago
Joined:
12 years, 11 months ago
Email:
m**********@gmail.com

I am a Forest Engineer that have interest in Genic Expression from Plant-Pathogen interactions. I live in Brazil and teaching in a University Universidade Federal dos Vales do Jequitinhonha e Mucuri, at Minas Gerais State.
http://www.ufvjm.edu.br

Posts by Marcelo Laia

<prev • 47 results • page 1 of 5 • next >
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Is possible to use NOISeq with counts from RSubread?
... I read in the NOISeq Users Guide that it use counts from TopHat. I have some trouble to use TopHat with my raw data: it is paired data. So, I ask if is possible to run RSubread, got a lot of files (I use gappedIndex=TRUE) and I think that NOISeq use only one index. Please, is it possible to you prov ...
rsubread noiseq tophat bowtie bowtie2 written 6 months ago by Marcelo Laia430
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Clustering large dataset
... I'm trying to do a MetaboAnalystR [1]'s analysis with a large dataset. All works great except PlotHeatMap function. This functions plot two type of image output: "overview" and "detail". In "overview" mode, we can do plot the image in png or pdf. However, in this mode, we do not could see the heatma ...
heatmap seqplots cairo graphics device failed to create cairo backend written 9 months ago by Marcelo Laia430 • updated 9 months ago by shepherl ♦♦ 1.7k
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Comment: A: qRT-PCR Sample Maximization software analysis and proper experimental design adv
... Any suggestion? ...
written 4.6 years ago by Marcelo Laia430
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qRT-PCR Sample Maximization software analysis and proper experimental design advice
... Hello, I am learning about experimental qRT-PCR design and I found Hellemans et al., 2007 [1] and Rieu and Powers 2009 [2]. So, I made a experimental design for me [3]. However, I am not sure what package I could use to do the analysis afterwards. Have you any suggestion to me about: A. My exper ...
qpcr sample maximization analysis experimental design qrt-pcr written 4.6 years ago by Marcelo Laia430 • updated 4.6 years ago by Gordon Smyth39k
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Comment: C: timecourse package - Missing data
... 2010/2/10 Yu Chuan Tai : > Hi Marcelo, > > The current version of Timecourse is for longitudinal time course data only. > Based on what you described below, your study looked like a cross- sectional > one because time point samples were from different animals. That's, they > were i ...
written 9.9 years ago by Marcelo Laia430
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timecourse package - Missing data
... >From the timecourse vignete: "For version 1.0.0, missing time point samples are not allowed inmb.long() andmb.MANOVA(), while missing replicates are allowed for a subset of genes." Is this missing data only allowed for a subset of genes or for a whole replicate? We have a data set with 5 time ...
timecourse written 9.9 years ago by Marcelo Laia430 • updated 9.9 years ago by Yu Chuan Tai440
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Answer: A: problems in installing biomart
... 2009/11/11 Andreia Fonseca : > Dear Forum > checking for xml2-config... no Did you have libxml2-dev installed? > checking for curl-config... no Did you have libcurl-dev installed? I hope this help you! -- Marcelo Luiz de Laia Universidade do Estado de Santa Catarina UDESC - www.cav.u ...
written 10.1 years ago by Marcelo Laia430
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Comment: C: Most stable gene pairs in array experiment
... 2009/10/20 Michael Dondrup : > Hi, > > you can ?try something like this or use two for loops: > > >> apply (mygenes, 1 , function(row) { apply (mygenes, 1, function(x) { >> var(row-x) ?} ) } ) > > ? ? ? ?geneA ? geneB ? geneC ? geneD ? geneE > geneA 0.00000 0.0410 ...
written 10.2 years ago by Marcelo Laia430
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limma technical replicates and biological replicates in the same design
... Hi, On the limma usersguide I found the example: FileName Cy3 Cy5 File1 wt1 mu1 File2 wt1 mu1 File3 wt2 mu2 File4 wt2 mu2 where two wild-type and two mice from the same mutant strain are compared using two arrays for each pair of mice. > biolrep <- c(1, 1, 2, 2) > corfit <- duplicate ...
limma santa written 10.2 years ago by Marcelo Laia430 • updated 6 months ago by Gordon Smyth39k
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OFF TOPIC high-density SSU rRNA microarray
... Good afternoon, I?m planning to conduct a study aimed at identifying and, if possible, quantifying members of the gut microbial community in broiler chickens, using the microarrays technology. Actually I?m looking for a high-density SSU rRNA microarray for this purpose. Therefore, I would like t ...
microarray santa written 10.2 years ago by Marcelo Laia430

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