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Showing :
Normalization
•
reset
2
votes
2
replies
388
views
[Diffbind] Blacklist error: Error: Invalid Normalization and Unable to apply Blacklist/Greylist
DiffBind
Normalization
24 days ago • updated 23 days ago
Henry
▴ 10
1
vote
6
replies
433
views
DESeq2 step-by-step Normalization then DGE separately & Normalization methods and switchability
DESeq2
Normalization
updated 4 weeks ago by
Michael Love
41k • written 4 weeks ago by
RL
• 0
0
votes
2
replies
168
views
edgeR analysis: glm functions and offset matrix
tximport
edgeR
Normalization
4 weeks ago
Marianna
▴ 20
1
vote
4
replies
293
views
EDAseq to normalise for both gene-length and GC-content
norma
EDASeq
EDA
Normalization
6 weeks ago
samuel.channon
• 0
0
votes
0
replies
152
views
TCGAbiolinks STAD project 450k Methylation Beta Value data
MethylationArray
normalize450K
TCGAbiolinks
Normalization
MethylationArrayData
8 weeks ago • updated 6 weeks ago
Giovanni Calice
▴ 160
2
votes
1
reply
300
views
Normalization factors when there is a possible unidirectional expression
limma
Normalization
DifferentialExpression
updated 11 weeks ago by
ATpoint
★ 4.1k • written 11 weeks ago by
Shaimaa Gamal
• 0
2
votes
2
replies
410
views
Graphing DEseq2 Normalized Counts using ggplot2
DESeq2
Normalization
RNASeq
12 weeks ago
jackafiore
• 0
2
votes
3
replies
370
views
TMM normalization after RUVg correction
RUVSeq
limma
DifferentialExpression
edgeR
Normalization
updated 3 months ago by
James W. MacDonald
65k • written 3 months ago by
Shaimaa Gamal
• 0
1
vote
3
replies
581
views
Comparing relative expression of a conserved gene between distantly related species.
ComparativeGenomics
Normalization
RNASeq
DESeq2
written 3 months ago by
georgii.vdovin
• 0
0
votes
1
reply
299
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 3 months ago by
ATpoint
★ 4.1k • written 3 months ago by
Ömür Koray
• 0
2
votes
4
replies
588
views
Does EdgeR trimmed mean of M values (TMM) account for gene length?
Normalization
edgeR
RNASeq
3 months ago
Sabiha
▴ 20
1
vote
1
reply
417
views
DESeq2 Normalization for QC Samples
DESeq2
Normalization
updated 3 months ago by
Michael Love
41k • written 4 months ago by
phmai1148
• 0
2
votes
2
replies
635
views
DESeq2 median of ratios normalization instead of VST or rlog for ordinations?
DESeq2
medianofratiostransformation
StatisticalMethod
Normalization
dispersion
4 months ago
Linton
• 0
0
votes
0
replies
251
views
SomaticSignatures normalization for trinucleotide motifs in reference genome
Normalization
SomaticSignatures
5 months ago
vasily.dobrovolsky
• 0
0
votes
6
replies
858
views
DESeq2 Normalization with 4 Groups
Normalization
RNASeq
DESeq2
6 months ago
erika.maldonado-rosado
• 0
0
votes
0
replies
282
views
Dual species RNA-seq, Library size and normalization
Normalization
edgeR
RNASeq
DESeq2
6 months ago
Florian
• 0
1
vote
11
replies
1.0k
views
DESeq2 gene length normalisation
Normalization
DESeq2
updated 6 months ago by
Michael Love
41k • written 6 months ago by
cm15245
• 0
1
vote
4
replies
2.5k
views
How to process Nimblegen microarray .pair files to get normalized expression matrix
Microarray
Preprocessing
NimbleGen
Normalization
written 17 months ago by
Sarah
• 0
1
vote
5
replies
1.0k
views
Does voom normalization quantile create difference in DGE genes when distribution of patients is similar (no big differences in quantiles)?
limmaGUI
normalization
limma
voom
Normalization
updated 10 months ago by
Gordon Smyth
50k • written 10 months ago by
manuelsokolov
• 0
0
votes
3
replies
612
views
Normalization of different input materials
Normalization
DESeq2
updated 10 months ago by
ATpoint
★ 4.1k • written 10 months ago by
altuda
▴ 10
0
votes
2
replies
757
views
Can VST-transformed community composition data be expressed as relative abundance?
vst
relativeabundance
Normalization
DESeq2
11 months ago
dwas
• 0
5
votes
11
replies
3.0k
views
Visualization of negative log CPM values
Visualization
Normalization
ggplot2
edgeR
written 11 months ago by
Sabiha
▴ 20
0
votes
3
replies
713
views
Is the gene-specific PCR efficiency a serious concern for intrasample comparisons in RNA-Seq?
RNA-Seq
normalization
PCR_efficiency
bias
intrasample_comparison
updated 12 months ago by
James W. MacDonald
65k • written 12 months ago by
FedeXander
• 0
1
vote
2
replies
900
views
Filter by expression and keep probes in limma
R
filter
limma
Normalization
Microarray
12 months ago
Abdul
▴ 20
2
votes
2
replies
667
views
Question about transforming log expression values to antilog values in limma
IlluminaChip
limma
AffymetrixChip
Normalization
Microarray
13 months ago
Abdul
▴ 20
1
vote
2
replies
621
views
RNA-Seq normalization of pre and post treatment groups
Normalization
RNASeq
written 13 months ago by
Sarah
• 0
0
votes
2
replies
717
views
Scaling and normalization by deconvolution of SMART-Seq 2 data
scran
Clustering
SingleCell
Normalization
13 months ago
Assa Yeroslaviz
★ 1.5k
1
vote
6
replies
1.8k
views
can negative value microarray matrix data analysed by limma directly
limma
microarray
normalization
updated 9 months ago by
Gordon Smyth
50k • written 3.6 years ago by
linouhao
▴ 20
2
votes
3
replies
1.8k
views
deseq2 normalization and statistical method
normalization
deseq2
StatisticalMethod
updated 13 months ago by
ATpoint
★ 4.1k • written 13 months ago by
SH
• 0
1
vote
1
reply
994
views
deseq2 RLE normalization
RLE
normalization
deseq2
updated 13 months ago by
ATpoint
★ 4.1k • written 13 months ago by
SH
• 0
0
votes
1
reply
546
views
Using DESeq2 only for normalization
DESeq2
Normalization
updated 13 months ago by
ATpoint
★ 4.1k • written 13 months ago by
phmai1148
• 0
0
votes
1
reply
412
views
processing samples with significantly different counts
DESeq2
Normalization
updated 13 months ago by
ATpoint
★ 4.1k • written 13 months ago by
Anastasiia
• 0
0
votes
5
replies
1.2k
views
DESeq Normalization and miRNAs Filtering
DESeq2
Normalization
updated 13 months ago by
ATpoint
★ 4.1k • written 13 months ago by
phmai1148
• 0
2
votes
3
replies
894
views
Batch correction and Deseq2
DESeq2
WGCNA
Normalization
NormalyzerDE
BatchEffect
updated 13 months ago by
ATpoint
★ 4.1k • written 13 months ago by
suphamon.j
▴ 20
0
votes
1
reply
541
views
Normalization of RNA seq data before DESeq2 and PCA in case of strong batch effects
DESeq2
Normalization
sva
updated 14 months ago by
ATpoint
★ 4.1k • written 14 months ago by
marslena
• 0
3
votes
3
replies
803
views
What is cpmByGroup used for?
edgeR
Normalization
updated 14 months ago by
James W. MacDonald
65k • written 14 months ago by
EM
• 0
0
votes
2
replies
738
views
Using Multi-Dimensional Scaling (MDS) to produce a vector in order to account for patient bias when constructing DGE lists from RNA-seq datasets in R?
PrincipalComponent
StatisticalMethod
edgeR
MultidimensionalScaling
Normalization
14 months ago
rcreswell01
• 0
1
vote
1
reply
771
views
Library sizes of miRNA sequencing data and TMM normalization with Edge-R
edgeR
SequencingData
miRNA
TMM
Normalization
updated 15 months ago by
Gordon Smyth
50k • written 15 months ago by
tleona3
• 0
4
votes
2
replies
1.7k
views
Clarification of what DESeq2::estimateSizeFactors controlGenes does and when it should *not* be used
DESeq2
SpikeIn
Normalization
DifferentialExpression
updated 15 months ago by
ATpoint
★ 4.1k • written 15 months ago by
kalavattam
▴ 10
0
votes
1
reply
634
views
Micro Array GEO data analysis
GEOquery
MicroarrayData
Normalization
Microarray
NanoStringDiff
updated 15 months ago by
James W. MacDonald
65k • written 15 months ago by
Aleksandra
• 0
0
votes
1
reply
1.3k
views
How to use spike-in information (sequences from another species) with DESeq2::DESeq()
DESeq2
SpikeIn
Normalization
updated 15 months ago by
Michael Love
41k • written 15 months ago by
kalavattam
▴ 10
4
votes
7
replies
2.0k
views
How to normalize long-read RNA-seq data for comparison with short-reads
NanoporeRNASeq
LongRead
Normalization
ShortRead
IlluminaRNASeq
15 months ago
Bernardo
▴ 10
1
vote
0
replies
577
views
Problem with VSN: Error in vsnML(sv) : L-BFGS-B needs finite values of 'fn'
justvsn
vsn
Normalization
vsn2
Proteomics
16 months ago • updated 15 months ago
Arend
▴ 10
0
votes
0
replies
736
views
Trying to use a NanoString expression set with batch effects to do GSVA
GSVA
Normalization
BatchEffect
GSVAdata
NanoString
16 months ago
Zhijie
• 0
1
vote
0
replies
674
views
Use scran's normalization by deconvolution for each individual sample or over all samples
scran
Normalization
scRNA
BatchEffect
16 months ago
r_grau
▴ 10
3
votes
4
replies
1.6k
views
RiP RLE normalisation using spike-in peaks in DiffBind for ChIP-seq
DiffBind
SpikeIn
Normalization
ChIP-seq
csaw
16 months ago
spg
• 0
4
votes
8
replies
2.2k
views
Can we use rlog or vst data values in limma for differential expression analysis?
DESeq2
limma
Normalization
R
updated 11 months ago by
Gordon Smyth
50k • written 17 months ago by
Sabiha
▴ 20
0
votes
1
reply
633
views
TCGA RNAseq Normalization
normalization
RNASEQ
Normalization
TCGAutils
TCGA
updated 17 months ago by
ATpoint
★ 4.1k • written 17 months ago by
Deniz
• 0
0
votes
2
replies
994
views
Deseq2 and co-expression network normalization
DESeq2
Normalization
DifferentialExpression
r
updated 17 months ago by
ATpoint
★ 4.1k • written 17 months ago by
lola
• 0
2
votes
4
replies
3.5k
views
Possibility of using TPM or FPKM values in edgeR for differential expression analysis?
DESeq2
R
edgeR
Normalization
RNASeq
17 months ago
mohammedtoufiq91
▴ 10
50 results • Page
1 of 1
Recent ...
Replies
Comment: Why does GSEA on edgeR results for randomized samples give highly significant p-
by
Chris
• 0
Intent to delete when going home. Sorry for that!
Comment: Why does GSEA on edgeR results for randomized samples give highly significant p-
by
Gordon Smyth
50k
Please ask you own question. Inserting your own dataset into someone else's question like this is very impolite. I have already answered m…
Comment: package goseq seems to be not available on the latest version of R
by
Gordon Smyth
50k
limma most certainly is available. It installs in R 4.4.0 and Bioc 3.19 for all platforms without any issues. It has not had any period of …
Comment: package goseq seems to be not available on the latest version of R
by
Patrycja
▴ 20
limma package also isn't available.Is there any information when these packages will be available?
Comment: Why does GSEA on edgeR results for randomized samples give highly significant p-
by
Chris
• 0
Thanks Gordon for the advice!
Votes
Comment: Why does GSEA on edgeR results for randomized samples give highly significant p-
Answer: Why does GSEA on edgeR results for randomized samples give highly significant p-
Handling multiple differential expression comparisons
Answer: fgsea significant result
Answer: fgsea significant result
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