Dear flowCore maintainer, 

I am developing a package that involves writing and reading fcs files.  I included an example in my package (modified here for simplicity) :

library(flowCore)
dat = as.matrix(iris[,1:4])
dat = flowFrame(dat)
write.FCS(dat,"dat.fcs")
dat = read.FCS("dat.fcs")

I tested the example in multiple operation systems (using the flowCore 1.42.2) and did not encounter any error. However, when I submit the package to Bioconductor, the example failed due to an error in the last line of the code :

Parameter(s) $P1B not contained in 'x'
Parameter(s) $P2B not contained in 'x'
Parameter(s) $P3B not contained in 'x'
Parameter(s) $P4B not contained in 'x'

Do you have an idea why the example failed on Bioconductor server?  Does the flowFrame function automatically generates $P1B, $P2B...... ? Any suggestions how I can fix this error? 

Thank you!

Zicheng

I've pushed the fix to both bioconductor devel and release

see https://github.com/RGLab/flowCore/issues/85#issuecomment-328613970 for details