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fatemeh.kaveh
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@fatemehkaveh-14124
Last seen 7.2 years ago
Hi, I have generated my own annotation file for feature count, However I do not get any error in running but I get zero number which means none of the reads are aligned to my selected regions (created in annotation file). In IGV, I can see that reads are aligned to the selected regions. The annotation file is like this:
GeneID Chr Start End Strand
>HLA:HLA00627 chr6 1 786 +
>HLA:HLA00629 chr6 1 769 +
.....
Hi, you need to follow the posting guide (http://www.bioconductor.org/help/support/posting-guide/) when you ask a question. For example, you need to provide information such as versions of software tools you use, your scripts related to your question etc.
Regarding your featureCounts question, you need to provide a reproducible example otherwise we can not help you.