Question: comparison of expression between orthologous species
gravatar for laksharikrish
12 months ago by
laksharikrish0 wrote:


I am PhD student and im working on a non-model organism trying to understand the differences in expression patterns and differentially expressed genes between resistant and susceptible responses .

I have 3 species (quite similar) infected with two different strains of a pathogen, some are susceptible and others resistant? I want to mainly understand the genes involved in defense & susceptible responses. The approach was to compare the orthologous genes across the three species. now i have a table with the gene expression values for each replicates (*3) in different treatments and log2FCby comparing to the mock and treatment of each responses. 

Is there any idea that someone could provide on how do i select the top candidates for defense responses and the highyl differentially expressed genes during susceptible interaction from this table ??

any suggestions/inputs will be super helpful.


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