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hauken_heyken ▴ 80@hauken_heyken-13992
Last seen 7 months ago
Hey, I have the circular Ecoli genome, and I want to construct a GRanges object of all ORFs in the fasta file from ncbi.
I made a c++ function to do it, but of course since some of the orfs that starts just before the stop of the genome, and ends after the start, will make IRanges() constructor output error (negative widths not allowed), I want to something like in R where you can set the metacolumn of the GRangesobject to include isCircular() = vector(T,T,T,...),
Here is the sample from c++ and error:
#include <Rcpp.h> Function GRangesC("GRanges", Environment::namespace_env("GenomicRanges")); Function IRangesC("IRanges", Environment::namespace_env("IRanges")); std::vector<int> ORFdef = orfs_as_vector(fastaSeq); // <- get all orfs from fasta Seqnames.insert(Seqnames.end(), ORFdef.size() / 2, 1); strands.insert(strands.end(),ORFdef.size() / 2,"+"); return(GRangesC(wrap(Seqnames), IRangesC(wrap(result_value), wrap(result_value)),wrap(strands))); Error in find_ORFs : Evaluation error: solving row 149014: negative widths are not allowed.
In more simple terms, what I am saying is:
How do you allow negative widths on IRanges ?