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paul.alto
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@paulalto-11559
Last seen 5.2 years ago
A couple of weeks or months ago, a question was posted regarding DESeq2's behavior when dealing with samples with one extreme outlier. In that case, DESeq2 identified that gene as DE, although there was only 1 out of N samples with extreme counts.
Michael Love suggested a way to force DESeq2 to look at all replicates.
I've been searching for the post for a while using both Google and the forum search but I can't find it.
Can somebody post the link, or maybe repeat the explanation here?
Thank you.
Thanks for your reply, Michael. Here's an example of the case I have in mind. These are TPMs:
wt1 53.18
wt2 2.81
wt3 3.58
wt4 3.03
wt5 2.31
wt6 2.69
ko1 1.79
ko2 2.98
ko3 3.54
ko4 3.35
ko5 3.22
ko6 4.07
ko7 2.1
wt1 has an extremely high TPM compared to the other samples. DESeq2's adjusted P-value is 0.014 and log2 fold change = -3.3335.
So you are saying that the default outlier filtering isn’t working and that you want it to be more aggressive here at filtering the high value/count? What is the Cook’s distance for this gene? (See vignette for how to access).
When I use cooksCutoff this gene has an NA adjusted P-value. I may be misremembering the post I'm looking for, but I thought there was a way to still include these cases but make DESeq2 somehow consider all replicates individually.
In cases like this, the gene is simply excluded from analysis or can it be considered non-DE?
What is the full result row for this gene, using defaults?
baseMeanA 116.779010545804
baseMeanB 11.5170403274273
baseMean 60.099488120524
log2FoldChange -3.33358245938423
lfcSE 0.955682659416467
stat -3.48816882522457
pvalue 0.000486340890441655
padj 0.0138045444267906
I thought you said it had NA for padj? What code do you use to obtain an NA here?
Sorry, I replied too fast. I do get NA for both pvalue and padj using defaults (cooksCutoff = TRUE and independentFiltering = TRUE).
Try minReplicatesForReplace=6
Thanks for the suggestion, Mike. Now that gene has pvalue = 0.31 and padj = 0.58. Another gene that had the same problem also has higher padj.