If I want to perform multiple tests, for example, 3 Wald tests (different design matrices), and 2 LTR tests (different full and reduced design matrices) would I need to create 5 separate `dds` objects?
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If I want to perform multiple tests, for example, 3 Wald tests (different design matrices), and 2 LTR tests (different full and reduced design matrices) would I need to create 5 separate `dds` objects?
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From how I understand your question, the answer is 'yes',
Strictly speaking, you need a different object for each model fit, i.e., for each result of a call to the DESeq
function with different inputs, e.g. same data but different model formula, or different arguments for the argument test
.
You can call the function results
on the same object with different arguments (e.g., contrast
) to perform different tests.
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