We have been running offTargetAnalysis on a large set of sequences. For some of the sequences, we ran into the following 2 kinds of error messages:
Searching for gRNAs ... Error in .Call2("new_XStringSet_from_CHARACTER", ans_class, ans_elementType, : key 69 (char 'E') not in lookup table Calls: offTargetAnalysis ... XStringSet -> XStringSet -> .charToXStringSet -> .Call2 -> .Call
I checked the sequences affected by this error and did not see any 'E' in there. Could you please tell me what might have caused this error?
Building feature vectors for scoring ...
Calculating scores ...
Error in `$<-.data.frame`(`*tmp*`, score, value = c(0.0831847889825708, :
replacement has 405816 rows, data has 405817
Calls: offTargetAnalysis -> getOfftargetScore2 -> $<- -> $<-.data.frame
Could you please tell me what to do to correct this?
Thank you very much!