Hi,

I am new to ngs data analysis.

I performed a microRNA sequencing experiments, and I have to admit there are no biological replicates (therefore, it's for exploratory usage).

However, I performed the standard DESeq2 worklow for my data, with multiple different treatments, and one untreated sample, as a control.

But in the results table I get sometimes log2foldchange values of 234203249320.

Is there something obvious I did wrong?

Also, as I have the multiple different treatments, to be compared to one control, is it correct usage to perform all at once AND assign the untreated to the control sample using the "relevel" function?

Please let me know if and which parts of my code you need.

Thanks in advance!