expresso dies on list.files
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Kort, Eric ▴ 220
@kort-eric-1483
Last seen 6.1 years ago
United States
(I apologize for sending this query a second time, but I forgot to add a descriptive subject last time which I thought may have contributed to the lack of response.) I am running 64 bit R 2.0.1 on SUSE Linux, and despite successful use in the past, I have been recently been unable to normalize some affy data using expresso. I receive an error with list.files that I can not figure out. While list.files seems to generate the error below, if I explicitly call "list.files("./"), I receive no error. Here is my code and the resulting error and traceback: >AB <- ReadAffy(celfile.path="./") > >eset <- expresso(AB, bgcorrect.method="mas", normalize.method="constant", + pmcorrect.method="mas", summary.method="mas", verbose=T) background correction: mas normalization: constant PM/MM correction : mas expression values: mas background correcting...done. normalizing...Error in list.files(path, pattern, all.files, full.names, recursive) : invalid directory argument > > traceback() 7: list.files(...) 6: list.celfiles(celfile.path, full.names = TRUE) 5: AllButCelsForReadAffy(..., filenames = filenames, widget = widget, celfile.path = celfile.path, sampleNames = sampleNames, phenoData = phenoData, description = description) 4: ReadAffy(celfile.path = dir) 3: normalize(afbatch, normalize.method) 2: do.call("normalize", c(alist(afbatch, normalize.method), normalize.param)) 1: expresso(AB, bgcorrect.method = "mas", normalize.method = "constant", pmcorrect.method = "mas", summary.method = "mas", verbose = T) Can someone tell me why this is not working? Thank you, Eric Kort Van Andel Research Institute Grand Rapids, MI 49503 USA This email message, including any attachments, is for the so...{{dropped}}
affy affy • 804 views
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@james-w-macdonald-5106
Last seen 3 hours ago
United States
Kort, Eric wrote: > (I apologize for sending this query a second time, but I forgot to add a > descriptive subject last time which I thought may have contributed to > the lack of response.) > > I am running 64 bit R 2.0.1 on SUSE Linux, and despite successful use in > the past, I have been recently been unable to normalize some affy data > using expresso. I receive an error with list.files that I can not > figure out. While list.files seems to generate the error below, if I > explicitly call "list.files("./"), I receive no error. > > Here is my code and the resulting error and traceback: > > >>AB <- ReadAffy(celfile.path="./") Is this really the calling sequence you are using? The traceback looks really strange to me, as there is no reason I know of that ReadAffy() should be called directly after normalize(). I wonder if something is busted. You might want to upgrade, as your Rversion is over a year old now, as are your BioC packages (or at least they _should_ be - you haven't installed new BioC packages in an old R installation, have you?). A sessionInfo() would be very helpful here. Best, Jim >> >>eset <- expresso(AB, bgcorrect.method="mas", > > normalize.method="constant", > + pmcorrect.method="mas", summary.method="mas", verbose=T) > background correction: mas > normalization: constant > PM/MM correction : mas > expression values: mas > background correcting...done. > normalizing...Error in list.files(path, pattern, all.files, full.names, > recursive) : > invalid directory argument > >>traceback() > > 7: list.files(...) > 6: list.celfiles(celfile.path, full.names = TRUE) > 5: AllButCelsForReadAffy(..., filenames = filenames, widget = widget, > celfile.path = celfile.path, sampleNames = sampleNames, phenoData > = phenoData, > description = description) > 4: ReadAffy(celfile.path = dir) > 3: normalize(afbatch, normalize.method) > 2: do.call("normalize", c(alist(afbatch, normalize.method), > normalize.param)) > 1: expresso(AB, bgcorrect.method = "mas", normalize.method = "constant", > pmcorrect.method = "mas", summary.method = "mas", verbose = T) > > > Can someone tell me why this is not working? > > Thank you, > > Eric Kort > Van Andel Research Institute > Grand Rapids, MI 49503 > USA > This email message, including any attachments, is for the so...{{dropped}} > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor -- James W. MacDonald Affymetrix and cDNA Microarray Core University of Michigan Cancer Center 1500 E. Medical Center Drive 7410 CCGC Ann Arbor MI 48109 734-647-5623
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