Number of reads needed for Guide-Seq
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@jacques-ptremblay-15721
Last seen 4.1 years ago

 How many sequences do you recommend using to identify the off-targets with the Guide-Seq method?

Best regards

Jacques P. Tremblay

 

Answer from Dr Zhu:

It depends on how many offTargets you expect and whether you are interested in identifying low incidence of offTargets. We often sequence half to one million reads per sample.

sequencing • 392 views
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