I am using qsea to study the methylome in paediatric cancer. Since TCGA does not provide such calibration data, I have to do it myself. I mimic the tutorial example to create a Granges object contain all 450k probe data. After executing qseaSet=addEnrichmentParameters(qseaSet, enrichmentPattern="CpG", windowIdx=wd, signal=signal), I get following error:
Error in estimateEnrichmentLM(qs, windowIdx = windowIdx, signal = signal, : number of samples (20) does not match number of columns (6) in "signal".
I separately run the tutorial again and cross-check the variable "signal", their format is the same. I have no idea what is the number of columns(6) refer to. Could anyone help me with this problem?