Is there any way to blast my nucleotide sequence only against specific NCBI database, for example, refseq_rna?
Similarly, I'd like to limit my search to a specific organism/taxid.

As I understand, blastSequences() doesn't have these options now. Is there any other solution?

Thank you James! I have contacted NCBI support, and the solution turned out to be quite simple: besides
QUERY, their API supports a fieldENTREZ_QUERY. Unfortunately it is not included inannotatefor now, but I simply added&ENTREZ_QUERY=human%5borgn%5dstring to myQUERY, and this worked!