Yesterday I was walking through this tutorial and I was able to run every package, but when I tried to execute the script today I wasn't able to load libraries that I had already installed (e.g. BiocStyle and GenomicFeatures). The error that I keep getting is attached below. Thus far I've tried every suggestion on this thread but nothing has worked. Is there anyway to restore these packages in R 3.5.0?
> install.packages("BiocStyle", repos="https://bioconductor.org/biocLite.R", dependencies=TRUE) Installing package into ‘C:/Users/KimLab/Documents/R/win-library/3.5’ (as ‘lib’ is unspecified) Warning in install.packages : unable to access index for repository https://bioconductor.org/biocLite.R/src/contrib: cannot open URL 'https://bioconductor.org/biocLite.R/src/contrib/PACKAGES' Warning in install.packages : package ‘BiocStyle’ is not available (for R version 3.5.0) Warning in install.packages : unable to access index for repository https://bioconductor.org/biocLite.R/bin/windows/contrib/3.5: cannot open URL 'https://bioconductor.org/biocLite.R/bin/windows/contrib/3.5/PACKAGES' > sessionInfo() R version 3.5.0 (2018-04-23) Platform: x86_64-w64-mingw32/x64 (64-bit) Running under: Windows >= 8 x64 (build 9200) Matrix products: default locale: [1] LC_COLLATE=English_United States.1252 [2] LC_CTYPE=English_United States.1252 [3] LC_MONETARY=English_United States.1252 [4] LC_NUMERIC=C [5] LC_TIME=English_United States.1252 attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] BiocInstaller_1.30.0 loaded via a namespace (and not attached): [1] Rcpp_0.12.17 locfit_1.5-9.1 [3] lattice_0.20-35 assertthat_0.2.0 [5] digest_0.6.15 SingleCellExperiment_1.2.0 [7] mime_0.5 R6_2.2.2 [9] GenomeInfoDb_1.16.0 plyr_1.8.4 [11] stats4_3.5.0 ggplot2_2.2.1 [13] pillar_1.2.3 zlibbioc_1.26.0 [15] rlang_0.2.0 lazyeval_0.2.1 [17] data.table_1.11.2 S4Vectors_0.18.2 [19] Matrix_1.2-14 BiocParallel_1.14.1 [21] RCurl_1.95-4.10 munsell_0.4.3 [23] shiny_1.1.0 DelayedArray_0.6.0 [25] compiler_3.5.0 httpuv_1.4.3 [27] vipor_0.4.5 pkgconfig_2.0.1 [29] BiocGenerics_0.26.0 ggbeeswarm_0.6.0 [31] htmltools_0.3.6 tidyselect_0.2.4 [33] SummarizedExperiment_1.10.1 tibble_1.4.2 [35] gridExtra_2.3 GenomeInfoDbData_1.1.0 [37] edgeR_3.22.2 IRanges_2.14.10 [39] matrixStats_0.53.1 viridisLite_0.3.0 [41] dplyr_0.7.5 later_0.7.2 [43] bitops_1.0-6 grid_3.5.0 [45] xtable_1.8-2 gtable_0.2.0 [47] magrittr_1.5 scales_0.5.0 [49] XVector_0.20.0 viridis_0.5.1 [51] promises_1.0.1 bindrcpp_0.2.2 [53] limma_3.36.1 DelayedMatrixStats_1.2.0 [55] tools_3.5.0 Biobase_2.40.0 [57] glue_1.2.0 beeswarm_0.2.3 [59] purrr_0.2.4 parallel_3.5.0 [61] yaml_2.1.19 colorspace_1.3-2 [63] GenomicRanges_1.32.3 shinydashboard_0.7.0 [65] bindr_0.1.1
Yes I do still get errors:
Install the missing dependency
BiocInstaller::biocLite("stringi")
; it's not really clear why stringi is (no longer) installed.