Search
Question: help for package DESeq2 in R
0
gravatar for ndiayediop2
4 weeks ago by
ndiayediop20 wrote:

hi 

i would like a help please

dds=DESeqDataSetFromMatrix(count = count,colData = colData,design = ~condition)

Error in DESeqDataSet(se, design = design, ignoreRank) : 
  some values in assay are negative

ADD COMMENTlink modified 29 days ago by Levi Waldron600 • written 4 weeks ago by ndiayediop20
0
gravatar for Levi Waldron
29 days ago by
Levi Waldron600
CUNY Graduate School of Public Health and Health Policy, New York, NY
Levi Waldron600 wrote:

The error message seems clear: DESeq2 is intended for count data, and counts cannot be negative. You'll have to identify an appropriate statistical model and implementation for your data. This answer is necessarily vague because I don't know anything about your experiment or data. This online course (https://www.edx.org/xseries/data-analysis-life-sciences) and associated textbook (https://leanpub.com/dataanalysisforthelifesciences) introduce different linear models and their implementations in R/Bioconductor.

ADD COMMENTlink written 29 days ago by Levi Waldron600

thank you Levi Waldron.i will try after.

ADD REPLYlink written 29 days ago by ndiayediop20
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.2.0
Traffic: 174 users visited in the last hour