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Question: help for package DESeq2 in R
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gravatar for ndiayediop2
5 months ago by
ndiayediop20 wrote:

hi 

i would like a help please

dds=DESeqDataSetFromMatrix(count = count,colData = colData,design = ~condition)

Error in DESeqDataSet(se, design = design, ignoreRank) : 
  some values in assay are negative

ADD COMMENTlink modified 5 months ago by Levi Waldron790 • written 5 months ago by ndiayediop20
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gravatar for Levi Waldron
5 months ago by
Levi Waldron790
CUNY Graduate School of Public Health and Health Policy, New York, NY
Levi Waldron790 wrote:

The error message seems clear: DESeq2 is intended for count data, and counts cannot be negative. You'll have to identify an appropriate statistical model and implementation for your data. This answer is necessarily vague because I don't know anything about your experiment or data. This online course (https://www.edx.org/xseries/data-analysis-life-sciences) and associated textbook (https://leanpub.com/dataanalysisforthelifesciences) introduce different linear models and their implementations in R/Bioconductor.

ADD COMMENTlink written 5 months ago by Levi Waldron790

thank you Levi Waldron.i will try after.

ADD REPLYlink written 4 months ago by ndiayediop20
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