Hello,

Using flowcore functions, I would like to keep columns containing raw data in a flowset after applying a transformation, example:

logicleT <- logicleTransform( w = 0.5, t= 10000, m =4.5) transformation <- transformList(c("FL2-A", "FL3-A", "FL6-A", "FL10-A"), logicleT ) newFlowSet <- transform(flowset, transformation)

If done as above, newFlowSet will contain flowframes with columns with transformed data and not the original data.

Is it possible at all to retain the original columns and add new columns with the transformed values?

Many thanks.

You can do inline transformation,e.g.

newFlowSet <- transform(flowset, `trans-FL2-A` = logicleT(`FL2-A`), `trans-FL3-A` = logicleT(`FL3-A`))

By the way, why do you need the original columns?