Hi, I have 5 samples, namely the following: (1) Not transfected, untreated (2) Transfected but untreated (3) Transfected, treated, analyzed after 5 mins (4) Transfected, treated, analyzed after 60 mins (5) Transfected, treated, analyzed after 4 hrs [By transfected I mean a particular vector is present, and treated means treated with an antibody]
I do not have any replicates for any of the conditions. I am looking to perform an Anova test using edgeR, to see the gene expression at different time points wrt sample 2 (as given).
Could anyone tell me the edgeR code I should run to do the test? Normally, I define the groups, normalize the library, create a design matrix with the group and normalized DGEList, followed by estimating dispersion and performing the GLMQL tests. I am confused as to how to proceed in this case,as I have no replicates. I don't know how to define the groups or estimate dispersion in this case.
I would be very grateful if someone could help me with the edgeR codes. Thank you