Question: Normalisation upstream rlog
0
gravatar for zhang.jianhai
6 months ago by
zhang.jianhai0 wrote:

Hello,

In the RNA Seq count data, upstream rlog normalisation should there be other normalisations like RPKM, CPM, etc.? Or the rlog is directly applied on raw count data?

Regards.

deseq2 rlog • 132 views
ADD COMMENTlink modified 6 months ago by Michael Love25k • written 6 months ago by zhang.jianhai0
Answer: Normalisation upstream rlog
0
gravatar for Michael Love
6 months ago by
Michael Love25k
United States
Michael Love25k wrote:

Raw counts or estimated counts from eg Salmon. See the transformation section of the workflow.

ADD COMMENTlink written 6 months ago by Michael Love25k

Can you share the link of the workflow that contains the transformation section?

Regards.

ADD REPLYlink written 6 months ago by zhang.jianhai0

It’s linked in the first page of the vignette.

ADD REPLYlink written 6 months ago by Michael Love25k
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