Question: z-score with DESeq2?
0
gravatar for andrebolerbarros
4 months ago by
andrebolerbarros10 wrote:

Hello everyone,

Do you know if it's possible to use Z-score values for counts normalized using DESeq2? My main objective is to get mean values of gene expression for several populations, mainly for visualization objectives.

Thanks in advance,

André

deseq2 • 141 views
ADD COMMENTlink modified 4 months ago by Michael Love25k • written 4 months ago by andrebolerbarros10
Answer: z-score with DESeq2?
1
gravatar for Michael Love
4 months ago by
Michael Love25k
United States
Michael Love25k wrote:

We have two transformations in DESeq2 for producing log scale data, see here:

https://bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html#the-variance-stabilizing-transformation-and-the-rlog

ADD COMMENTlink written 4 months ago by Michael Love25k

Thanks Michael.

So, the procedures would be to perform Z-score on the rlog-transformed data?

André

ADD REPLYlink written 27 days ago by andrebolerbarros10

Yes or the VST data.

ADD REPLYlink written 27 days ago by Michael Love25k
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