z-score with DESeq2?
1
0
Entering edit mode
@andrebolerbarros-16788
Last seen 10 hours ago
Portugal

Hello everyone,

Do you know if it's possible to use Z-score values for counts normalized using DESeq2? My main objective is to get mean values of gene expression for several populations, mainly for visualization objectives.

Thanks in advance,

André

deseq2 • 25k views
ADD COMMENT
1
Entering edit mode
@mikelove
Last seen 7 hours ago
United States

We have two transformations in DESeq2 for producing log scale data, see here:

https://bioconductor.org/packages/release/workflows/vignettes/rnaseqGene/inst/doc/rnaseqGene.html#the-variance-stabilizing-transformation-and-the-rlog

ADD COMMENT
0
Entering edit mode

Thanks Michael.

So, the procedures would be to perform Z-score on the rlog-transformed data?

André

ADD REPLY
0
Entering edit mode

Yes or the VST data.

ADD REPLY

Login before adding your answer.

Traffic: 692 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6