I am importing a FlowJo Workspace via parsing the workspace, and then normalizing the workspace using the normalize() function. No issues here. However, after taking these steps, I am trying to export the files via write.flowSet. But when I do so, the data comes back untransformed, so the MFI is squished between 50-200 or so, instead of between -500 and 26,000 as I would expect.
I have tried nearly every which way of utilizing the flowJobiexponential transform function but it just isn't working right. For example, it transforms the data from MFI 70 to MFI 700, when it should really be MFI in the 1000s. Does anyone know how to inverse transform the data? It works when I use getStats and set inverse.transform = TRUE, but I can't find a similar function when writing the transformed FCS files. I wish there were something like, write.flowSet(inverse.transform=TRUE). Would be so helpful.