DESeq2 Wald test p-values
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Homer • 0
@homer-18328
Last seen 4.2 years ago

I don't understand how DESeq2 calculates the p-values given by DESeq2::results(). I thought it was

2 * pnorm(abs(log2FoldChange / lfcSE), lower.tail = FALSE)

with log2FoldChange and lfcSE taken from the corresponding columns of the output table given by DESeq2::results(). However, when calculating the p-values this way, I get non-negligible differences (i.e. not only numerical inaccuracies) to the p-values given by DESeq2.

deseq2 • 514 views
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Entering edit mode
Homer • 0
@homer-18328
Last seen 4.2 years ago

Sorry, just realized my mistake: I was using a threshold for the log fold change.

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