Question: rlog in reference level
0
gravatar for Morris
7 days ago by
Morris0
Morris0 wrote:

Hello everyone,

I analyzed RNA-seq data and I noticed that the rlog value of the reference level for some genes are lower then the rest of the samples, how can the Log2FC be negative if the reference level has lower compared to other samples?

Thank you

deseq2 • 44 views
ADD COMMENTlink modified 7 days ago by swbarnes2310 • written 7 days ago by Morris0
Answer: rlog in reference level
2
gravatar for swbarnes2
7 days ago by
swbarnes2310
swbarnes2310 wrote:

rlog values aren't used in calculating log fold changes. Size normalized values are.

ADD COMMENTlink written 7 days ago by swbarnes2310
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