Differential gene expression analysis
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rthapa • 0
@rthapa-22163
Last seen 4.4 years ago

Hi,

I am using DESeq2 to do differential gene expression analysis. I have two tolerant lines and two susceptible lines. I am wondering if I consider two susceptible lines as reference level to find DEGs, does DESeq2 take average of the gene counts in two susceptible lines and estimate DEGs?

Thanks

deseq2 • 312 views
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@mikelove
Last seen 21 hours ago
United States

Take a look at the 2014 DESeq2 paper which talks through how the model works.

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