Hello, I would like to ask a question about CyTOF. I have a set of files from multiple acquisition periods of the same pool of barcoded samples (14 clinical samples and 1 reference samples). We couldn't measure everything in one go because of, well, clogging and stuff. Here is the ridgeline plot of CD3 expression distribution across acquisitions.

https://ibb.co/X2NbjWc

I do not think there's a significant variation of marker expression distribution across acquisitions. What do you think? Would it be acceptable to just concatenate all samples and then debarcode?

Thanks in advance for your advice.