I am performing differential gene expression using DESeq2 but will be subsetting transcripts based on a pre-determined criterion. Does this sub-setting violate any assumptions make by the statistical package? In other words, does DESeq2 expect a minimum number of genes? Thanks!

This has been asked before on the site but I'm sure it's very difficult to find the answer.

I found it by google with site:support.bioconductor.org deseq2 subset genes

See my answer here:

https://support.bioconductor.org/p/86113/#86396