Question: Cell cycle analysis of FACS data
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gravatar for Ulrik Stervbo
13.4 years ago by
Ulrik Stervbo80 wrote:
Hello everyone, This may be more of a general R question, but since there is some biology involved, I post it here. I did already post the question on the Purdue Cytometry mailing list without any luck. I am looking to analyse cell cycle data (percent cells in the G1, S and G2 phase, as well as sub-G1 population), obtained by FACS analysis of PI stained cells, using R on a linux machine. Does anyone know if I have to implement a solution myself, and if so, is there any advice i need? I am thinking about implementing algorithm used in Cylchred (if I can backtrack it form "This utility is based on Watson's Pragmatic Model (Watson et al 1987 Cytometry 8:1-8) with modifications by Ormerod 1991 and Hoy 1996") though I would prefer a solution which could also analyse aneuploidy. I am reading the FCS files in R using rflowcyt. Thanks in advance for any help and suggestions Ulrik
rflowcyt cycle • 1.1k views
ADD COMMENTlink written 13.4 years ago by Ulrik Stervbo80
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