Question: BioC normalisations for small array 2 colour data?
13.0 years ago by
Dan Swan • 50
Dan Swan • 50 wrote:
Hi, I have some data from a small specialised microarray - 200 genes, 1 spiked control, 1 negative control. This is 2 colour data, with dye swaps. I was wondering what an appropriate normalisation for this scenario is within Bioconductor given that Lowess is unreliable for <1000 genes? Any pointers would be gratefully recieved. thanks, Dan -- Senior Research Associate, Bioinformatics Support Unit, Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Framlington Place, University of Newcastle upon Tyne, Newcastle, NE2 4HH Tel: +44 (0)191 222 7253 (Leech offices: Rooms M.2046/M.2046A) Tel: +44 (0)191 246 4833 (Devonshire offices: Rooms G.25/G.26) Website: http://bioinf.ncl.ac.uk/support/
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