SNPRMA: Normalizing...Error in readChar(con, 5) : cannot open the connection
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@alex-lisovich-2232
Last seen 9.6 years ago
Dear All, I am getting the following error when using snprma() on oligo v.1.0.2-6 with pd.mapping250k.sty which I believe was once discussed on the list on June 4, 2007 (see below): >library(oligo) >library(pd.mapping250k.sty) >rawData<-read.celfiles(list.celfiles()) >rma<-snprma(rawData) > Normalizing...Error in readChar(con, 5) : cannot open the connection > In addition: Warning message: > cannot open compressed file I am wondering if this problem was solved and if yes what was a solution. Thank you in advance for your help! Alex >Hi An, > >that's problably R complaining a file that it didn't find. The >strange thing is that I can't reproduce it here. Could you please >forward me the results of traceback()? > >This would happen if, for example, oligo finds the old version of the >pd.mapping250k.sty package. > >b On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: >> Hi Ben, >> >> When using oligo version 1.0.2-3, the pmStrand function indeed >> works :-) >> but the snprma function results in an error: >> >> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >> rmaSty <- snprma(fsetSty) >> >> Normalizing...Error in readChar(con, 5) : cannot open the connection >> In addition: Warning message: >> cannot open compressed file >> >> How can I check which connection needs to be established? >> >> Thanks in advance for your help! >> An >>
oligo oligo • 1.2k views
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@benilton-carvalho-1375
Last seen 4.1 years ago
Brazil/Campinas/UNICAMP
What are your sessionInfo() and traceback()? If you installed the pd.mapping pkgs from the source, make sure you're using the latest version. If your OS is windows, we're working on a solution for the pd.mapping pkgs. b On Jun 23, 2007, at 10:56 AM, Alex Lisovich wrote: > Dear All, > > I am getting the following error when using snprma() on oligo > v.1.0.2-6 with pd.mapping250k.sty which I believe was once discussed > on the list on June 4, 2007 (see below): > >> library(oligo) >> library(pd.mapping250k.sty) >> rawData<-read.celfiles(list.celfiles()) >> rma<-snprma(rawData) > >> Normalizing...Error in readChar(con, 5) : cannot open the connection >> In addition: Warning message: >> cannot open compressed file > > I am wondering if this problem was solved and if yes what was a > solution. > > Thank you in advance for your help! > > Alex > > > > >> Hi An, >> >> that's problably R complaining a file that it didn't find. The >> strange thing is that I can't reproduce it here. Could you please >> forward me the results of traceback()? >> >> This would happen if, for example, oligo finds the old version of the >> pd.mapping250k.sty package. >> >> b > > On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > >>> Hi Ben, >>> >>> When using oligo version 1.0.2-3, the pmStrand function indeed >>> works :-) >>> but the snprma function results in an error: >>> >>> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >>> rmaSty <- snprma(fsetSty) >>> >>> Normalizing...Error in readChar(con, 5) : cannot open the connection >>> In addition: Warning message: >>> cannot open compressed file >>> >>> How can I check which connection needs to be established? >>> >>> Thanks in advance for your help! >>> An >>> > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/ > gmane.science.biology.informatics.conductor
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Benilton, The OS is Mac OSX Tiger on dual-core intel MacPro. The pd.mapping250k.sty was built from Affymetris .cdf, .csv and probe_tab files using pdInfoBuilder and installed through R -arch=x86_64 CMD INSTALL. The sessioInfo() and traceback(): > sessionInfo() R version 2.5.0 Patched (2007-05-02 r41420) x86_64-apple-darwin8.9.1 locale: C attached base packages: [1] "splines" "tools" "stats" "graphics" "grDevices" "utils" [7] "datasets" "methods" "base" other attached packages: pd.mapping250k.sty oligo BufferedMatrixMethods "0.1.5" "1.0.2-6" "1.0.0" BufferedMatrix RSQLite DBI "1.0.0" "0.5-4" "0.2-3" affyio Biobase "1.4.0" "1.14.0" > > traceback() 3: readChar(con, 5) 2: load(system.file("extdata", paste(annot, "Ref.rda", sep = ""), package = annot)) 1: snprma(rawData) > Thank you, Alex On 6/23/07, Benilton Carvalho <bcarvalh at="" jhsph.edu=""> wrote: > What are your sessionInfo() and traceback()? > > If you installed the pd.mapping pkgs from the source, make sure > you're using the latest version. > > If your OS is windows, we're working on a solution for the pd.mapping > pkgs. > > b > > On Jun 23, 2007, at 10:56 AM, Alex Lisovich wrote: > > > Dear All, > > > > I am getting the following error when using snprma() on oligo > > v.1.0.2-6 with pd.mapping250k.sty which I believe was once discussed > > on the list on June 4, 2007 (see below): > > > >> library(oligo) > >> library(pd.mapping250k.sty) > >> rawData<-read.celfiles(list.celfiles()) > >> rma<-snprma(rawData) > > > >> Normalizing...Error in readChar(con, 5) : cannot open the connection > >> In addition: Warning message: > >> cannot open compressed file > > > > I am wondering if this problem was solved and if yes what was a > > solution. > > > > Thank you in advance for your help! > > > > Alex > > > > > > > > > >> Hi An, > >> > >> that's problably R complaining a file that it didn't find. The > >> strange thing is that I can't reproduce it here. Could you please > >> forward me the results of traceback()? > >> > >> This would happen if, for example, oligo finds the old version of the > >> pd.mapping250k.sty package. > >> > >> b > > > > On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > > > >>> Hi Ben, > >>> > >>> When using oligo version 1.0.2-3, the pmStrand function indeed > >>> works :-) > >>> but the snprma function results in an error: > >>> > >>> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) > >>> rmaSty <- snprma(fsetSty) > >>> > >>> Normalizing...Error in readChar(con, 5) : cannot open the connection > >>> In addition: Warning message: > >>> cannot open compressed file > >>> > >>> How can I check which connection needs to be established? > >>> > >>> Thanks in advance for your help! > >>> An > >>> > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: http://news.gmane.org/ > > gmane.science.biology.informatics.conductor >
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The pd.mapping packages contain metadata information not available on the Affymetrix files. So, use this instead: http://www.bioconductor.org/packages/2.0/data/annotation/html/ pd.mapping250k.sty.html b On Jun 25, 2007, at 11:46 AM, Alex Lisovich wrote: > Benilton, > > The OS is Mac OSX Tiger on dual-core intel MacPro. > > The pd.mapping250k.sty was built from Affymetris .cdf, .csv and > probe_tab files using pdInfoBuilder and installed through R > -arch=x86_64 CMD INSTALL. > > The sessioInfo() and traceback(): > >> sessionInfo() > R version 2.5.0 Patched (2007-05-02 r41420) > x86_64-apple-darwin8.9.1 > > locale: > C > > attached base packages: > [1] "splines" "tools" "stats" "graphics" "grDevices" > "utils" > [7] "datasets" "methods" "base" > > other attached packages: > pd.mapping250k.sty oligo BufferedMatrixMethods > "0.1.5" "1.0.2-6" "1.0.0" > BufferedMatrix RSQLite DBI > "1.0.0" "0.5-4" "0.2-3" > affyio Biobase > "1.4.0" "1.14.0" >> > >> traceback() > 3: readChar(con, 5) > 2: load(system.file("extdata", paste(annot, "Ref.rda", sep = ""), > package = annot)) > 1: snprma(rawData) >> > > Thank you, > > Alex > > On 6/23/07, Benilton Carvalho <bcarvalh at="" jhsph.edu=""> wrote: >> What are your sessionInfo() and traceback()? >> >> If you installed the pd.mapping pkgs from the source, make sure >> you're using the latest version. >> >> If your OS is windows, we're working on a solution for the pd.mapping >> pkgs. >> >> b >> >> On Jun 23, 2007, at 10:56 AM, Alex Lisovich wrote: >> >> > Dear All, >> > >> > I am getting the following error when using snprma() on oligo >> > v.1.0.2-6 with pd.mapping250k.sty which I believe was once >> discussed >> > on the list on June 4, 2007 (see below): >> > >> >> library(oligo) >> >> library(pd.mapping250k.sty) >> >> rawData<-read.celfiles(list.celfiles()) >> >> rma<-snprma(rawData) >> > >> >> Normalizing...Error in readChar(con, 5) : cannot open the >> connection >> >> In addition: Warning message: >> >> cannot open compressed file >> > >> > I am wondering if this problem was solved and if yes what was a >> > solution. >> > >> > Thank you in advance for your help! >> > >> > Alex >> > >> > >> > >> > >> >> Hi An, >> >> >> >> that's problably R complaining a file that it didn't find. The >> >> strange thing is that I can't reproduce it here. Could you please >> >> forward me the results of traceback()? >> >> >> >> This would happen if, for example, oligo finds the old version >> of the >> >> pd.mapping250k.sty package. >> >> >> >> b >> > >> > On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: >> > >> >>> Hi Ben, >> >>> >> >>> When using oligo version 1.0.2-3, the pmStrand function indeed >> >>> works :-) >> >>> but the snprma function results in an error: >> >>> >> >>> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) >> >>> rmaSty <- snprma(fsetSty) >> >>> >> >>> Normalizing...Error in readChar(con, 5) : cannot open the >> connection >> >>> In addition: Warning message: >> >>> cannot open compressed file >> >>> >> >>> How can I check which connection needs to be established? >> >>> >> >>> Thanks in advance for your help! >> >>> An >> >>> >> > >> > _______________________________________________ >> > Bioconductor mailing list >> > Bioconductor at stat.math.ethz.ch >> > https://stat.ethz.ch/mailman/listinfo/bioconductor >> > Search the archives: http://news.gmane.org/ >> > gmane.science.biology.informatics.conductor >>
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Benilton, Now it's working. Thank you very much! Alex On 6/25/07, Benilton Carvalho <bcarvalh at="" jhsph.edu=""> wrote: > The pd.mapping packages contain metadata information not available on > the Affymetrix files. > > So, use this instead: > > http://www.bioconductor.org/packages/2.0/data/annotation/html/ > pd.mapping250k.sty.html > > b > > On Jun 25, 2007, at 11:46 AM, Alex Lisovich wrote: > > > Benilton, > > > > The OS is Mac OSX Tiger on dual-core intel MacPro. > > > > The pd.mapping250k.sty was built from Affymetris .cdf, .csv and > > probe_tab files using pdInfoBuilder and installed through R > > -arch=x86_64 CMD INSTALL. > > > > The sessioInfo() and traceback(): > > > >> sessionInfo() > > R version 2.5.0 Patched (2007-05-02 r41420) > > x86_64-apple-darwin8.9.1 > > > > locale: > > C > > > > attached base packages: > > [1] "splines" "tools" "stats" "graphics" "grDevices" > > "utils" > > [7] "datasets" "methods" "base" > > > > other attached packages: > > pd.mapping250k.sty oligo BufferedMatrixMethods > > "0.1.5" "1.0.2-6" "1.0.0" > > BufferedMatrix RSQLite DBI > > "1.0.0" "0.5-4" "0.2-3" > > affyio Biobase > > "1.4.0" "1.14.0" > >> > > > >> traceback() > > 3: readChar(con, 5) > > 2: load(system.file("extdata", paste(annot, "Ref.rda", sep = ""), > > package = annot)) > > 1: snprma(rawData) > >> > > > > Thank you, > > > > Alex > > > > On 6/23/07, Benilton Carvalho <bcarvalh at="" jhsph.edu=""> wrote: > >> What are your sessionInfo() and traceback()? > >> > >> If you installed the pd.mapping pkgs from the source, make sure > >> you're using the latest version. > >> > >> If your OS is windows, we're working on a solution for the pd.mapping > >> pkgs. > >> > >> b > >> > >> On Jun 23, 2007, at 10:56 AM, Alex Lisovich wrote: > >> > >> > Dear All, > >> > > >> > I am getting the following error when using snprma() on oligo > >> > v.1.0.2-6 with pd.mapping250k.sty which I believe was once > >> discussed > >> > on the list on June 4, 2007 (see below): > >> > > >> >> library(oligo) > >> >> library(pd.mapping250k.sty) > >> >> rawData<-read.celfiles(list.celfiles()) > >> >> rma<-snprma(rawData) > >> > > >> >> Normalizing...Error in readChar(con, 5) : cannot open the > >> connection > >> >> In addition: Warning message: > >> >> cannot open compressed file > >> > > >> > I am wondering if this problem was solved and if yes what was a > >> > solution. > >> > > >> > Thank you in advance for your help! > >> > > >> > Alex > >> > > >> > > >> > > >> > > >> >> Hi An, > >> >> > >> >> that's problably R complaining a file that it didn't find. The > >> >> strange thing is that I can't reproduce it here. Could you please > >> >> forward me the results of traceback()? > >> >> > >> >> This would happen if, for example, oligo finds the old version > >> of the > >> >> pd.mapping250k.sty package. > >> >> > >> >> b > >> > > >> > On Jun 4, 2007, at 8:07 AM, De Bondt, An-7114 [PRDBE] wrote: > >> > > >> >>> Hi Ben, > >> >>> > >> >>> When using oligo version 1.0.2-3, the pmStrand function indeed > >> >>> works :-) > >> >>> but the snprma function results in an error: > >> >>> > >> >>> fsetSty <- read.celfiles(dir()[grep("S.CEL", dir())]) > >> >>> rmaSty <- snprma(fsetSty) > >> >>> > >> >>> Normalizing...Error in readChar(con, 5) : cannot open the > >> connection > >> >>> In addition: Warning message: > >> >>> cannot open compressed file > >> >>> > >> >>> How can I check which connection needs to be established? > >> >>> > >> >>> Thanks in advance for your help! > >> >>> An > >> >>> > >> > > >> > _______________________________________________ > >> > Bioconductor mailing list > >> > Bioconductor at stat.math.ethz.ch > >> > https://stat.ethz.ch/mailman/listinfo/bioconductor > >> > Search the archives: http://news.gmane.org/ > >> > gmane.science.biology.informatics.conductor > >> >
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