I am working with biological replicates and I am a bit worried about the biological variation between samples.
For example, the abundance of a certain gene in sample 1 could be hundreds of time higher or lower than in sample B. If this is the case, this will significantly affect the P-value in the t-test.
As such, my question is whether there is a way we can account for this fact in the statistical analysis?
I will be much grateful if you guys could shed some light on this topic?