**320**wrote:

Hi all

I am working with biological replicates and I am a bit worried about the biological variation between samples.

For example, the abundance of a certain gene in sample 1 could be hundreds of time higher or lower than in sample B. If this is the case, this will significantly affect the P-value in the t-test.

As such, my question is whether there is a way we can account for this fact in the statistical analysis?

I will be much grateful if you guys could shed some light on this topic?

Thank you

Yogi

**33k**• written 10.7 years ago by Yogi Sundaravadanam •

**320**